Optimization of jackfruit seed starch hydrolysis process using glucoamylase: Response surface methodology approach
Article Sidebar
Main Article Content
Optimization of jackfruit seed starch hydrolysis process using glucoamylase: Response surface methodology approach
Abstract
Jackfruit seeds, an underutilized by-product of jackfruit processing, are rich in starch and represent a promising raw material for bioconversion into fermentable sugars. This study investigated the enzymatic hydrolysis of jackfruit seed starch using glucoamylase to produce reducing sugars. The effects of incubation temperature and reaction time were initially evaluated using the one-factor-at-a-time (OFAT) approach, followed by optimization through response surface methodology (RSM) with a central composite design (CCD). The optimal hydrolysis conditions were 70.75°C of incubation temperature and 4.8 h of reaction time, resulting in the highest reducing sugar concentration of 9.286 ± 0.228 mg/mL. The findings highlight the potential of using jackfruit seeds as a substrate for the enzymatic hydrolysis to produce fermentable sugars, contributing to sustainable waste valorization and bioethanol production.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
- Blanco, C.A., Caballero, I., Barrios, R., Rojas, A. (2014). Innovations in the brewing industry: Light beer. Int. J. Food Sci. Nutr., 65, 655–660.
- Carrasco, M., Alcaíno, J., Cifuentes, V., Baeza, M. (2017). Purification and characterization of a novel cold-adapted fungal glucoamylase. Microb. Cell Fact., 16, 75.
- Doan, C.T., Tran, T.N., Nguyen, A.D., Wang, S.-L. (2024). Enzymatic production of chitooligosaccharide using a GH Family 46 chitosanase from Paenibacillus elgii and its antioxidant activity. Catalysts, 14, 761.
- Doan, C. T., Tran, T. N., Wang, S.-L. (2021). Production of thermophilic chitinase by Paenibacillus sp. TKU052 by bioprocessing of chitinous fishery wastes and its application in N-acetyl-D-glucosamine production. Polymers, 13, 3048.
- Guan, X., Yao, H. (2008). Optimization of viscozyme L-assisted extraction of oat bran protein using response surface methodology. Food Chemistry, 106, 345–351.
- Hồ, T.H., Lê, C.L.C., Nguyễn, T.A.T., Nguyễn, T.N.H. (2022). Ảnh hưởng của các điều kiện chiết tách đến tính chất của tinh bột hạt mít Thái Lá Bàng (Artocarpus heterophyllus Lam.). Tạp chí Khoa học Trường Đại học Tây Nguyên, 55, 16–22.
- Kim, J., Kim, D., Ryu, K.H., Enhancing response surface methodology through coefficient clipping based on prior knowledge. Processes, 11, 3392.
- Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426–428.
- Nuriana, W., Wuryantoro. (2015). Ethanol synthesis from jackfruit (Artocarpus heterophyllus Lam.)
- stone waste as renewable energy source. Energy Procedia. 65, 372–377.
- Phạm, T.M.T., Nguyễn, T.P.H., Cao, D.K., Trần, D.L., Vo, G.L., Nguyễn, T.D.T., Trần, D.V. (2016). Khảo sát một số yếu tố ảnh hưởng lên quá trình thủy phân tinh bột hạt mít thành nguyên liệu sản xuất ethanol. Tạp chí Khoa học TDMU, 3, 19–26.
- Robinson, PK. (2015). Enzymes: principles and biotechnological applications. Essays Biochem., 59, 1–41.
- Tong, L., Zheng, J., Wang, X., Huang, H., Yang, H., Tu, t., Wang, Y., Bai, Y., Yao, B., Luo, H., Qin, Xing. (2021). Improvement of thermostability and catalytic efficiency of glucoamylase from Talaromyces leyettanus JCM12802 via site-directed mutagenesis to enhance industrial saccharification applications. Biotechnol. Biofuels, 14, 202.
- Xian, L., Feng, J. (2018). Purification and biochemical characterization of a novel mesophilic glucoamylase from Aspergillus tritici WZ99. Int. J. Biol. Macromol. 107, 1122–1130.
- Wang, C., Yang, L., Luo, L., Tang, S., Wang, Q. (2020). Purification and characterization of glucoamylase of Aspergillus oryzae from Luzhou-flavour Daqu. Biotechnol. Lett., 42, 2345–2355.