Functional domain characteristics and sequence variation of the NANB protein of Pasteurella multocida type D isolated from buffaloes in Dak Lak province and comparison with type B strains
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Functional domain characteristics and sequence variation of the NANB protein of Pasteurella multocida type D isolated from buffaloes in Dak Lak province and comparison with type B strains
Abstract
In the context of limited molecular data on Pasteurella multocida type D isolated from buffaloes in Dak Lak province, this study analyzed the neuraminidase NanB protein of a type D strain, designated EH32, and compared it with two type B strains, PM248 and the Iranian strain used for vaccine production. NanB sequences were extracted from whole–genome sequencing data; signal peptides were predicted using SignalP–5.0, domain architecture was annotated using InterProScan, and amino acid variation was assessed by multiple sequence alignment. The results showed that NanB from all three strains contained a Sec/SPI signal peptide with a conserved cleavage site at position 25/26 (TFG|QI), and shared a domain architecture consisting of an N–terminal sialidase region and a C–terminal β–barrel autotransporter domain. Within the scope of comparison with the two type B strains, PM248 and Iran, NanB from EH32 exhibited an overall identity of 87.2% across directly comparable sites. Variation was mainly concentrated in the passenger/sialidase region, with a PID of 76.2% and several short indels, whereas the autotransporter region was highly conserved, with a PID of 97.7%. Within the scope of the present dataset, the sequence variations observed in NanB from EH32 were predominantly localized to the passenger/sialidase region. This finding highlights this region as a priority target for further investigation of its potential roles in sialidase activity, antigenicity, and mucosal adaptation through 3D structural modeling and functional assays.
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Nguyễn Văn, T., Trịnh Thị Thu, H., Nguyễn Ngọc, Đỉnh, Nguyễn Văn, T., & Vũ Khắc, H. (2026). Functional domain characteristics and sequence variation of the NANB protein of Pasteurella multocida type D isolated from buffaloes in Dak Lak province and comparison with type B strains. Tay Nguyen Journal of Sciences, 20(3), 15–26. Retrieved from https://tckhtn.ttn.edu.vn/index.php/tckh/article/view/726
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References
- Almagro A.J.J. et al. (2019). SignalP 5.0 improves signal peptide predictions using deep neural networks. Nature Biotechnology, 37(4), 420–423. https://doi.org/10.1038/s41587-019-0036-z
- Blum, M. et al. (2025). InterPro: The protein sequence classification resource in 2025. Nucleic Acids Research, 53(D1), D444–D456. https://doi.org/10.1093/nar/gkae1082
- Cao, X., et al. (2024). Pathogenicity and Genomic Characteristics Analysis of Pasteurella multocida Serotype A Isolated from Argali Hybrid Sheep. Microorganisms, 12(6), 1072. https://doi.org/10.3390/MICROORGANISMS12061072
- Clarke, K.R. et al. (2022). Phylogenetic Classification and Functional Review of Autotransporters. Frontiers in Immunology, 13(July), 1–20. https://doi.org/10.3389/fimmu.2022.921272
- De Alwis, M.C.L. (1999). Haemorrhagic septicaemia. In Australian Centre for International Agricultural Research (ACIAR) (Vol. 57). Brown Prior Anderson, Melbourne.
- Ewers, C. et al. (2006). Virulence genotype of Pasteurella multocida strains isolated from different hosts with various disease status. Veterinary Microbiology, 114(3–4), 304–317. https://doi.org/10.1016/j.vetmic.2005.12.012
- Gharibi, D. et al. (2016). Isolation, molecular characterization and antibiotic susceptibility pattern of Pasteurella multocida isolated from cattle and buffalo from Ahwaz, Iran. Archives of Razi Institute, 72(2), 93–100. https://doi.org/10.22092/ari.2017.109838
- Hall, T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
- Harper, M. et al. (2006). Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiology Letters, 265(1), 1–10. https://doi.org/10.1111/J.1574-6968.2006.00442.X
- Harris D.B. (2010). Type V Secretion: the Autotransporter and Two-Partner Secretion Pathways. EcoSal Plus, 4(1), 1–30. https://doi.org/10.1053/j.gastro.2019.03.013.
- Jilani, M., et al. (2022). Elucidating the Structural Impacts of Protein InDels. Biomolecules, 12(10), 1435. https://doi.org/10.3390/BIOM12101435
- Johnson, T.J. et al. (2013). Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity. BMC Microbiology, 13(1). https://doi.org/10.1186/1471-2180-13-106
- Jones, P. et al. (2014). InterProScan 5: Genome-scale protein function classification. Bioinformatics, 30(9), 1236–1240. https://doi.org/10.1093/bioinformatics/btu031
- Käll, L. et al. (2004). A combined transmembrane topology and signal peptide prediction method. Journal of Molecular Biology, 338(5), 1027–1036. https://doi.org/10.1016/j.jmb.2004.03.016
- Kearse, M. et al. (2012). Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28(12), 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
- Leo, J.C. et al. (2012). Type V secretion: Mechanism(S) of autotransport through the bacterial outer membrane. Philosophical Transactions of the Royal Society B: Biological Sciences, 367(1592), 1088–1101. https://doi.org/10.1098/rstb.2011.0208
- Lestari, T.D. et al. (2025). Hemorrhagic septicemia: A major threat to livestock health. Open Veterinary Journal, 15(2), 519–532. https://doi.org/10.5455/OVJ.2025.V15.I2.3
- Luo, Y. et al. (1999). Sequence analysis of Pasteurella multocida major outer membrane protein (OmpH) and application of synthetic peptides in vaccination of chickens against homologous strain challenge. Vaccine, 17(7–8), 821–831. https://doi.org/10.1016/S0264-410X(98)00266-7
- Miton, C.M., and Tokuriki, N. (2022). Insertions and Deletions (Indels): A Missing Piece of the Protein Engineering Jigsaw. Biochemistry, 62(2), 148–157. https://doi.org/10.1021/ACS.BIOCHEM.2C00188
- Mizan, S. et al. (2000). Cloning and characterization of sialidases with 2-6′ and 2-3′ sialyl lactose specificity from Pasteurella multocida. Journal of Bacteriology, 182(24), 6874–6883. https://doi.org/10.1128/JB.182.24.6874-6883.2000
- Nugroho, C.M.H. et al. (2022). Screening and purification of NanB sialidase from Pasteurella multocida with activity in hydrolyzing sialic acid Neu5Acα2-6Gal and Neu5Acα2-3Gal. Scientific Reports, 12, 9681. https://doi.org/10.1038/s41598-022-13635-x
- Nguyen, T.V. et al. (2025). Comprehensive capsular, lipopolysaccharide, virulence, and antimicrobial resistance profiling of Pasteurella multocida isolated from buffaloes in Vietnam: First report of capsular type D and predominant L2 genotype. Veterinary World, 18(12), 4069–4081. https://doi.org/10.14202/vetworld.2025.4069-4081
- R Core Team. (2025). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/
- Sarangi, L.N. et al. (2014). Virulence genotyping of Pasteurella multocida isolated from multiple hosts from India. Scientific World Journal, 2014, 1–10. https://doi.org/10.1155/2014/814109
- Seemann, T. (2014). Prokka: rapid prokaryotic genome annotation. Bioinformatics, 30(14), 2068–2069. https://doi.org/10.1093/BIOINFORMATICS/BTU153
- Shayegh, J. et al. (2010). Potential of Pasteurella multocida isolated from healthy and diseased cattle and buffaloes in induction of diseases. Bulletin of the Veterinary Institute in Pulawy, 54(3), 299–304.
- Tesfaye, A.B. et al. (2025). Advances in Pasteurella multocida Vaccine Development: From Conventional to Next-Generation Strategies. Vaccines, 13(10), 1–20. https://doi.org/10.3390/vaccines13101034
- Thompson, J.D. et al. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673–4680. https://doi.org/10.1093/nar/22.22.4673
- Topolska, M. et al. (2025). Deep indel mutagenesis reveals the impact of amino acid insertions and deletions on protein stability and function. Nature Communications 2025 16:1, 16(1), 2617-. https://doi.org/10.1038/s41467-025-57510-5
- Wick, R.R. et al. (2017). Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLOS Computational Biology, 13(6), e1005595. https://doi.org/10.1371/JOURNAL.PCBI.1005595
- WOAH. (2021). Haemorrhagic Septicaemia (Pasteurella multocida serotypes 6:B and 6:E). In OIE Terrestrial Manual 2021 (pp. 1–16).
- DTU Health Tech (n.d.) SignalP–5.0: Signal peptide and cleavage sites in gram+, gram- and eukaryotic amino acid sequences. Available at: https://services.healthtech.dtu.dk/services/SignalP-5.0/ (Accessed: 22 September 2025).
- EMBL–EBI (n.d.) InterPro. Available at: https://www.ebi.ac.uk/interpro/ (Accessed: 22 September 2025).