Enhancement of Prodigiosin production from soybean residue by-product via fermentation technology
Article Sidebar
Main Article Content
Enhancement of Prodigiosin production from soybean residue by-product via fermentation technology
Abstract
Prodigiosin (PG) is a red pigment compound originating from Serratia marcescens. Recently, this compound has been extensively studied for its production process and investigation of its potential bioactivities. However, most previous studies used commercial nutrient broth as a carbon/nitrogen (C/N) source for cultivation in small-scale flasks. Regarding the cost-effective and green production, we reused soybean residue by-product (SRBP) for the biosynthesis of PG on a large scale using a 14-L bioreactor system and reported its potent anti-nematode activity. The experimental results revealed that Serratia marcescens TNU2 produced PG at the highest yield (5700 mg/L) under fermentation conditions: 6 L of liquid medium containing 1.75% C/N (SRBP/casein at the ratio of 8/2), 0.05% MgSO4, 0.1% K2HPO4, with an initial pH of 7.0, and the fermentation was performed at 27.5°C in 10 h. On the activity tests, the purified PG at the tested concentration of 1 mg/mL demonstrated a potential nematicidal effect against root-knot nematode on both J2 nematodes (100%) and eggs hatching (87.43%) at 1 and 3 days after treatment with PG, respectively. This study suggested that SRBP is a good C/N source for the bioproduction of PG with potential use for managing root-knot nematodes.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
- Aruldass, C.A.; Venil, C.K.; Zakaria, Z.A.; Ahmad, W.A. Brown sugar as a low-cost medium for the production of prodigiosin by locally isolated Serratia marcescens UTM1. Int. Biodeterior. Biodegrad. 2014, 95, 19-24.
- Bhagwat, A.; Padalia, U. Optimization of prodigiosin biosynthesis by Serratia marcescens fusing unconventional bioresources. J. Genet. Eng. Biotechnol. 2020, 18, 26.
- Chenqiang, L.; Xianbo, J.; Yu, F.; Longjun, C.; Hui, Z.; Rongbin, L.; Jichen, C. Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets. Electron. J. Biotechnol. 2019, 40, 58-64.
- Giri, A.V.; Anandkumar, N.; Muthukumaran, G.; Pennathur, G. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol. 2004, 4, 11.
- Gohil, N., Bhattacharjee, G., Kalariya, R. et al. Biovalorization of agro-industrial waste soybean meal for the production of prodigiosin by Serratia marcescens. Biomass Conv. Bioref. 2021. https://doi.org/10.1007/s13399-021-02102-8.
- Khan, Z.; Kim, S.G.; Jeon, Y.H.; Khan, H.U.; Son, S.H.; Kim, Y.H. A plant growth promoting rhizobacterium, Paenibacillus polymyxastrain GBR-1, suppresses root-knot nematode. Bioresour. Technol. 2008, 99, 3016–3302.
- Nguyen, T.H.; Wang, S.-L.; Doan, M.D.; Nguyen, T.H.; Tran, T.H.T.; Tran, T.N.; Doan, C.T.; Ngo, V.A.; Ho, N.D.; Do, V.C.; Nguyen, A.D.; Nguyen, V.B. Utilization of By-Product of Groundnut Oil Processing for Production of Prodigiosin by Microbial Fermentation and Its Novel Potent Anti- Nematodes Effect. Agronomy 2022a, 12, 41.
- Nguyen, T.H.; Wang, S.L.; Nguyen, D.N.; Nguyen, A.D.; Nguyen, T.H.; Doan, M.D.; Ngo, V.A.; Doan, C.T.; Kuo, Y.H.; Nguyen, V.B. Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin. Molecules 2021, 26, 3138.
- Nguyen, T.H.; Wang, S.-L.; Nguyen, T.H.; Doan, M.D.; Tran, T.H.T.; Ngo, V.A.; Ho, N.D.; Tran, T.N.; Doan, C.T.; Do, V.C.; Nguyen, A.D.; Nguyen, V.B. Utilization of Fishery-Processing By-Product Squid Pens for Scale-Up Production of Phenazines via Microbial Conversion and Its Novel Potential Antinematode Effect. Fishes 2022b, 7, 113.
- Nguyen, V.B.; Chen, S.P.; Nguyen, T.H.; Nguyen, M.T.; Tran, T.T.T.; Doan, C.T.; Tran, T.N.; Nguyen, A.D.; Kuo, Y.H.; Wang, S.L. Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer PG. Marine Drugs 2020, 18, 15.
- Nguyen, V.B.; Wang, S.-L.; Nguyen, A.D.; Phan, T.Q.; Techato, K.; Pradit, S. Bioproduction of Prodigiosin from Fishery Processing Waste Shrimp Heads and Evaluation of Its Potential Bioactivities. Fishes 2021, 6, 30.
- Sumathi, C.; Mohana, P.D.; Swarnalatha, S.; Dinesh, M.G.; Sekaran, G. Production of prodigiosin using tannery fleshing and evaluating its pharmacological effects. Sci World J. 2014, 4, 290327.
- Wang, S.L.; Nguyen, V.B.; Doan, C.T.; Tran, T.N.; Nguyen, M.T.; Nguyen, A.D. Production and potential applications of bioconversion of chitin and protein-containing fishery byproducts into prodigiosin: A review. Molecules 2020, 25, 2744.
- Wei, Y.H.; Chen, W.C. Enhanced production of prodigiosin-like pigment from Serratia marcescens SMdeltaR by medium improvement and oil-supplementation strategies. J. Biosci. Bioeng. 2005, 99, 616-622.